| PCR Sequencing Protocols 1996 Edition Contributor(s): Rapley, Ralph (Editor) |
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ISBN: 1489940383 ISBN-13: 9781489940384 Publisher: Humana OUR PRICE: $104.49 Product Type: Paperback - Other Formats Published: August 2013 |
| Additional Information |
| BISAC Categories: - Science | Life Sciences - Cell Biology - Science | Life Sciences - Molecular Biology - Medical | Laboratory Medicine |
| Dewey: 574.873 |
| Series: Methods in Molecular Biology |
| Physical Information: 0.5" H x 6" W x 9" (0.71 lbs) 221 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: Advances in bioscience research usually arise as a result of the continu- ing refinement of existing technologies. However, there are a number of occa- sions v rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu- tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech- nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac- tion. This technique, first reported in 1985 by MuUis and his colleagues, pro- vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase. |