| Monoclonal Antibody Protocols 1995 Edition Contributor(s): Davis, William C. (Author) |
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ISBN: 0896033082 ISBN-13: 9780896033085 Publisher: Humana OUR PRICE: $104.49 Product Type: Hardcover - Other Formats Published: May 1995 |
| Additional Information |
| BISAC Categories: - Science - Medical | Immunology |
| Dewey: 616.079 |
| LCCN: 95013504 |
| Series: Methods in Molecular Biology |
| Physical Information: 0.87" H x 6.52" W x 9.01" (0.97 lbs) 264 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: Since the initial description of techniques to immortalize anti- body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species. Stable hybrids can be readily produced in mice using a number of myeloma and hybridoma cell lines. To obviate the problem of identifying fusion partners in other animal species, xenohybrids have been produced using B-lymphocytes from the relevant species and mouse myeloma cells. The use of xenohybrids has minimized the problem of obtain- ing stable antibody-producing hybrids in all species examined thus far. Although alternative techniques are being developed to produce monoclonal antibodies by molecular methods, hybridoma technol- ogy will remain the technology of choice for producing monoclonal antibodies for a variety of applications in research and industry. The objective of Monoclonal Antibody Protocols is to provide investigators with a set of methods for producing and using mono- clonal antibodies in biomedical, agricultural, and biological sciences. The book is not intended to provide methodology for all possible applications, but rather a series of methods presented in an easy-- follow format that can be used by new and established investiga- tors, graduate and postgraduate fellows, and technical staff. |