| Molecular Analysis of Cancer 2002 Edition Contributor(s): Boultwood, Jacqueline (Editor), Fidler, Carrie (Editor) |
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ISBN: 0896036227 ISBN-13: 9780896036222 Publisher: Humana OUR PRICE: $161.49 Product Type: Hardcover - Other Formats Published: October 2001 Annotation: Leading researchers describe in step-by-step detail their best state-of-the art molecular techniques for elucidating the biochemical processes associated with human cancer. Among the techniques presented for identifying chromosomal rearrangements are fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), microsatellite analysis, PCR and RT-PCR, and DNA fingerprinting. Additional methods include SSCP, DGGE, the nonisotopic RNase cleavage assay, and the protein truncation assay and DNA sequencing for identifying mutations. |
| Additional Information |
| BISAC Categories: - Medical | Oncology - General - Medical | Genetics - Medical | Pathology |
| Dewey: 616.994 |
| LCCN: 2001024306 |
| Series: Methods in Molecular Medicine |
| Physical Information: 0.9" H x 6.32" W x 9.24" (1.41 lbs) 304 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: Over the past 20 years, technological advances in molecular biology have proven invaluable to the understanding of the pathogenesis of human cancer. The application of molecular technology to the study of cancer has not only led to advances in tumor diagnosis, but has also provided markers for the assessment of prognosis and disease progression. The aim of Molecular Ana- sis of Cancer is to provide a comprehensive collection of the most up-to-date techniques for the detection of molecular changes in human cancer. Leading researchers in the field have contributed chapters detailing practical pro- dures for a wide range of state-of-the-art techniques. Molecular Analysis of Cancer includes chapters describing techniques for the identification of chromosomal abnormalities and comprising: fluor- cent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), and microsatellite analysis. FISH has a pro- nent role in the molecular analysis of cancer and can be used for the detection of numerical and structural chromosomal abnormalities. The recently described SKY, in which all human metaphase chromosomes are visualized in specific colors, allows for the definition of all chromosomal rearrangements and marker chromosomes in a tumor cell. Protocols for the detection of chromosomal re- rangements by PCR and RT-PCR are described, as well as the technique of DNA fingerprinting, a powerful tool for studying somatic genetic alterations in tumorigenesis. |