| Genomic Imprinting: Methods and Protocols 2002 Edition Contributor(s): Ward, Andrew (Editor) |
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ISBN: 089603741X ISBN-13: 9780896037410 Publisher: Humana OUR PRICE: $104.49 Product Type: Hardcover - Other Formats Published: October 2001 Annotation: Andrew Ward and a team of experienced researchers have brought together a collection of optimized classic and vanguard techniques for the identification and analysis of imprinted genes. Protocols are included for identifying and cloning imprinted genes, for analyzing imprinted gene expression, for the study of DNA methylation and methylation-sensitive DNA-binding proteins, and for examining chromatin structure. There are also methods for the manipulation of mouse embryos to produce monoparental embryos and embryonic stem cells, and for the generation of transgenic mice with BAC, PAC, and YAC constructs. Each technique is described in step-by-step detail to ensure successful results. |
| Additional Information |
| BISAC Categories: - Science | Life Sciences - Molecular Biology - Science | Life Sciences - Genetics & Genomics - Science | Research & Methodology |
| Dewey: 572.860 |
| LCCN: 2001024456 |
| Series: Methods in Molecular Biology |
| Physical Information: 0.88" H x 6" W x 9" (1.52 lbs) 377 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: Genomic imprinting is the process by which gene activity is regulated according to parent of origin. Usually, this means that either the maternally inherited or the paternally inherited allele of a gene is expressed while the opposite allele is repressed. The phenomenon is largely restricted to mammals and flowering plants and was first recognized at the level of whole genomes. Nuclear transplantation experiments carried out in mice in the late 1970s established the non-equivalence of the maternal and paternal genomes in mammals, and a similar conclusion was drawn from studies of interploidy crosses of flowering plants that extend back to at least the 1930s. Further mouse genetic studies, involving animals carrying balanced translocations (reviewed in Chapter 3), indicated that imprinted genes were likely to be widely scattered and would form a minority within the mammalian genome. The first imprinted genes were identified in the early 1990s; over forty are now known in mammals and the list continues steadily to expand. |