| Morphology Methods: Cell and Molecular Biology Techniques 2001 Edition Contributor(s): Lloyd, Ricardo V. (Editor) |
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ISBN: 0896039552 ISBN-13: 9780896039551 Publisher: Humana OUR PRICE: $161.49 Product Type: Hardcover - Other Formats Published: June 2001 Annotation: Ricardo Lloyd, md, phd, provides critical and tutorial surveys of the cutting-edge molecular and cell biologic techniques used today in the analysis of cells and tissues in situ. These molecular-based histologic and morphologic techniques are described in step-by-step detail by hands-on experts, often those who have originated or perfected them. Among the techniques presented are laser capture microdissection, in situ hybridization, FISH, confocal laser scanning microscopy, in situ PCR, and tyramide amplification. Applications include the detection of infectious agents and DNA, the diagnosis of undifferentiated tumors and endocrine lesions, the metaphase analysis of whole blood cultures, and clonality analysis. In its integrated, state-of-the-art approach, Morphology Methods: Cell and Molecular Biology Techniques thoroughly details and applies the rich panoply of significant new cell and molecular biologic technologies to all the major problems confronting morphologic studies and diagnostic pathology today. |
| Additional Information |
| BISAC Categories: - Science | Research & Methodology - Science | Life Sciences - Cell Biology - Science | Life Sciences - Molecular Biology |
| Dewey: 571.602 |
| LCCN: 2001016959 |
| Physical Information: 1" H x 7" W x 10" (2.20 lbs) 422 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: The past several decades have witnessed an impressive array of conceptual and techno- logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula- tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab- th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under- standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure. |
