| Microrna Profiling: Methods and Protocols Softcover Repri Edition Contributor(s): Rani, Sweta (Editor) |
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ISBN: 1493982257 ISBN-13: 9781493982257 Publisher: Humana OUR PRICE: $104.49 Product Type: Paperback - Other Formats Published: June 2018 |
| Additional Information |
| BISAC Categories: - Medical | Genetics - Science | Life Sciences - Biochemistry |
| Dewey: 572.88 |
| Series: Methods in Molecular Biology |
| Physical Information: 0.55" H x 7" W x 10" (1.01 lbs) 246 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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