| Atomic Force Microscopy: Biomedical Methods and Applications 2004 Edition Contributor(s): Braga, Pier Carlo (Editor), Ricci, Davide (Editor) |
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ISBN: 1588290948 ISBN-13: 9781588290946 Publisher: Humana OUR PRICE: $161.49 Product Type: Hardcover Published: October 2003 Annotation: Highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection. |
| Additional Information |
| BISAC Categories: - Medical | Biochemistry - Science | Microscopes & Microscopy - Science | Life Sciences - Biochemistry |
| Dewey: 502.82 |
| LCCN: 2003015100 |
| Series: Methods in Molecular Biology |
| Physical Information: 1.09" H x 6.26" W x 8.98" (1.95 lbs) 394 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e., photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called "far-field microscopes" because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs). |