| Application of DNA Microarray Technology for Wastewater Analysis Contributor(s): Berthiaume, Fridiric (Author), Brousseau, Roland (Author), Lemarchand, Karine (Author) |
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ISBN: 1843397277 ISBN-13: 9781843397274 Publisher: Water Environment Research Foundation OUR PRICE: $159.60 Product Type: Paperback Published: July 2005 * Not available - Not in print at this time *Annotation: Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. |
| Additional Information |
| BISAC Categories: - Technology & Engineering | Chemical & Biochemical - Technology & Engineering | Environmental - General - Science | Applied Sciences |
| Dewey: 628.1 |
| LCCN: 2004106881 |
| Series: Werf Report |
| Physical Information: 0.23" H x 8.25" W x 11" (0.60 lbs) 102 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. |