| Fed-Batch Fermentation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli Contributor(s): Moulton, G. G. (Author) |
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ISBN: 1907568921 ISBN-13: 9781907568923 Publisher: Woodhead Publishing OUR PRICE: $194.75 Product Type: Hardcover - Other Formats Published: October 2014 |
| Additional Information |
| BISAC Categories: - Medical | Biotechnology - Science | Chemistry - Organic - Science | Biotechnology |
| Dewey: 547.29 |
| Series: Woodhead Publishing Series in Biomedicine |
| Physical Information: 0.5" H x 6.14" W x 9.21" (0.97 lbs) 188 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies. |