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This textbook covers the basic aspects of stem cell research and applications in regenerative medicine. Each chapter includes a didactic component and a practical section. The book offers readers insights into:

• How to identify the basic concepts of stem cell biology and the molecular regulation of pluripotency and stem cell development.
• How to produce induced pluripotent stem cells (iPSCs) and the basics of transfection.
• The biology of adult stem cells, with particular emphasis on mesenchymal stromal cells and hematopoietic stem cells, and the basic mechanisms that regulate them.
• How cancer stem cells arise and metastasize, and their properties.
• How to develop the skills needed to isolate, differentiate and characterize adult stem The clinical significance of stem cell research and the potential problems that need to be overcome.
• Evaluating the use of stem cells for tissue engineering and therapies (the amniotic membrane)
• The applications of bio-nanotechnology in stem cell research.
• How epigenetic mechanisms, including various DNA modifications and histone dynamics, are involved in regulating the potentiality and differentiation of stem cells.
• The scientific methods, ethical considerations and implications of stem cell research.