| Basil Proteomics Contributor(s): Sharma, Vikas (Author), Singh Jadon, Vikash (Author) |
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ISBN: 3659235695 ISBN-13: 9783659235696 Publisher: LAP Lambert Academic Publishing OUR PRICE: $50.27 Product Type: Paperback Published: September 2012 |
| Additional Information |
| BISAC Categories: - Science | Life Sciences - Biology |
| Physical Information: 0.19" H x 6" W x 9" (0.28 lbs) 80 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: As a part of the systematic and proteomic approach based characterization of plant material with the advantage of producing valuable informations of functional genomics for a general crop improvement programme, leaves of holy medicinal-aromatic plant Tulsi (Ocimum basilicum L.) were used as a source of proteins and a model eukaryotic system. In this study, we developed a reliable protocol for PEG mediated enrichment of low abundant cellular proteins to increase their detection through 2 DE-SDS PAGE. I6% PEG was found to be optimum for the precipitation of Ocimum protein. Further, proteome proteins in different fractions were analysed electrophoretically including 2- DE technique. These protocols may also will helpful in further studies in basil proteomics and other plants also. |