| Plant DNA Infectious Agents Softcover Repri Edition Contributor(s): Hohn, Thomas (Editor), Schell, Josef (Editor) |
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ISBN: 3709174589 ISBN-13: 9783709174586 Publisher: Springer OUR PRICE: $104.49 Product Type: Paperback - Other Formats Published: October 2011 |
| Additional Information |
| BISAC Categories: - Science | Life Sciences - Molecular Biology - Science | Life Sciences - Cell Biology - Science | Life Sciences - Botany |
| Dewey: 581.873 |
| Series: Plant Gene Research |
| Physical Information: 0.76" H x 7" W x 10" (1.40 lbs) 348 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: There has been recent rapid progress in the transformation of plants with foreign DNA, making use either of the natural routes of genetic invasion that viruses and bacteria have developed, or of chemical, mechanical and electrical tricks to make plant protoplast membranes permeable to nucleic acids. Genes integrated into plant virus genomes can be carried systemi- cally from the initial site of infection into the rest of the plant. Genes placed between the borders of Agrobacterium tumefaciens T-DNA can be transferred into single cells or plant tissue, which then divides to produce wound calli, or as in the case of an Agrobacterium rhizogenes infection, grow out into new roots. Calli and roots can be grown into whole plants. If virus genomes are placed between the T-DNA borders, a very effective infectious route, termed "agroinfection", is established. Once inside a pro- toplast, DNA finds its way into the nucleus where it can finally integrate into the resident chromosome and be expressed. Whether it can also find its way into chloroplasts is not yet clear, but at least translation products can be targeted into this organelle. Regeneration of whole organisms from single cells is a special feature of plants and offers a unique tool to study genes in a multicellular organism. In addition, as in animal cells, transcription and translation of trans- forming genes can be studied in plant cells during "transient expression". |