| Comparative Study of Ivf and Parthenogenesis in Bubaline Oocytes Contributor(s): Mishra, Vivek (Author), K. Misra, A. (Author) |
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ISBN: 3844326960 ISBN-13: 9783844326963 Publisher: LAP Lambert Academic Publishing OUR PRICE: $60.53 Product Type: Paperback Published: April 2011 |
| Additional Information |
| BISAC Categories: - Medical | Veterinary Medicine - General |
| Physical Information: 0.33" H x 6" W x 9" (0.47 lbs) 140 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: The objective of this study was to understand the pitfalls of the poor oocyte cleavage and subsequent embryo production by assessing the developmental competence of the oocytes in buffalo using parthenogenesis as a model which was compared with IVF (natural activation). Buffalo ovaries were collected from slaughter house and matured oocytes were subjected to either IVF (control) or chemical activation (treatment) 7% ethanol (ET)for 7 min ] 2.5 mM 6-Di methyl amino purine (6-DMAP) for 4 hr, or 7% ET for 7 min + 10 ug/ml Cycloheximide (CHX) for 6 hr or 7% ET for 7 min + 2.5 mM 6-DMAP + 10 ug/ml CHX for 6 hr]. Fertilized and chemically activated oocytes were cultured in mSOF medium for 8 days to study embryo development.In conclusion, the results of parthenogenesis, revealed that buffalo oocytes had better inherent developmental competence and the poor cleavage and embryo development following IVF, in our study, may partly be due to the poor quality of frozen-thawed sperm, improper sperm capacitation and/ or fertilization. Further, high ambient temperature adversely affected the cleavage and embryo development following IVF. |