| Plant beta galactosidases Contributor(s): Amin, Shajrul (Author), Masood, Akbar (Author), Gulzar, Sadaf (Author) |
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ISBN: 3846512338 ISBN-13: 9783846512333 Publisher: LAP Lambert Academic Publishing OUR PRICE: $69.77 Product Type: Paperback Published: September 2011 |
| Additional Information |
| BISAC Categories: - Science | Life Sciences - Biochemistry |
| Physical Information: 0.38" H x 6" W x 9" (0.55 lbs) 164 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: β-galactosidases belong to glycosyl hydrolase family of enzymes.These enzymes are widely distributed in nature.β-galactosidases have been implicated in many biological functions like hydrolysis of dietary lactose, degradation of glycolipids and proteoglycans, fruit ripening, cell elongation, storage mobilisation and petal senescence. β - galactosidase has proven as a best alternative for elimination of lactose intolerance.The present study was aimed at investigating the biochemical properties of β-galactosidases from apricots.Three isoenzymes(β-gal I,β-gal II and β-gal III) were obtained by salt fractionation and ion-exchange and Sephadex G-100 column chromatography. β-gal I, II and III showed 0.39, 0.52 and 0.30 units of activity respectively. The molecular weight of β-gal I, II an III as determined by gel filtration was found to be 44.15,34.70 and 23.71 KDa respectively. The optimum pH for the activity of different isozymes was between 4 and 6. The isoenzymes were determined to be thermally stable upto 40oC. The Km for β-gal I was 1.85mM,1.7mM for β-gal II and 1.19 for β-gal III.The Vmax for β-gal I,β-gal II aand β-gal III was found to be 0.52,0.70 and 0.38 mole/min respectively. |
