| A pneumococcal protein glycocapture approach Contributor(s): Olaya Abril, Alfonso (Author) |
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ISBN: 6202101806 ISBN-13: 9786202101806 Publisher: Editorial Academica Espanola OUR PRICE: $36.83 Product Type: Paperback Language: Spanish Published: February 2018 |
| Additional Information |
| BISAC Categories: - Science | Life Sciences - Biochemistry |
| Physical Information: 0.16" H x 6" W x 9" (0.25 lbs) 68 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: Emergence of pneumococcal multi-drug resistant clinical isolates prompts the need for a more effective vaccine for the prevention of disease. For this purpose, glycoproteins could be excellent candidates because of their implication in a number of biological processes, such as cell-to-cell recognition and the host immune response. In this study, methods widely used to capture potential surface proteins independently of their glycosylation status were carried out, and Concanavalin A (ConA) and Hydrazide subproteomes were obtained from different pneumococcal subcellular fractions (membrane, lysate, secretome and membrane-derived vesicles (MVs)) followed by protein LC-MS/MS analysis. In addition, a 2DE multilectinblotting (2DEMLB) analysis, using ConA, WGA, UEA, BC-1 and PNA lectins, was performed. Alfonso Olaya Abril*, Lidia G mez Gasc n, Irene Jim nez Mungu a, Michael J. McConnell, Meritxell Garc a Quintanilla, Ignacio Obando Santaella, Manuel Jos Rodr guez Ortega. |