| Up-regulation of RhoB by Large Clostridial Cytotoxins Contributor(s): Hülsenbeck, Johannes (Author) |
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ISBN: 3639001680 ISBN-13: 9783639001686 Publisher: VDM Verlag Dr. Mueller E.K. OUR PRICE: $50.27 Product Type: Paperback Published: April 2008 |
| Additional Information |
| BISAC Categories: - Science | Life Sciences - Biochemistry |
| Physical Information: 0.14" H x 6" W x 9" (0.23 lbs) 68 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: Clostridium difficile toxins A (TcdA) and B (TcdB) are monoglucosyl-transferases that inactivate RhoA, Rac1, and Cdc42. By these means, the toxins cause actin reorganisation (cytopathic effect) and apoptosis (cytotoxic effect). The cytotoxic effect has generally been attributed to the inactivation of RhoA. Treatment of cultured cells with Clostridium difficile toxin A (TcdA) also causes RhoB up-regulation. This study focuses on the up-regulation of RhoB and its functional consequences in toxin-treated cells. RhoB is up-regulated after treatment of fibroblasts with RhoA/B/C (TcdB, C. limosum C3) or H/K/N-Ras (C. sordellii lethal toxin) inactivating toxins. The up-regulation is based on transcriptional activation. p38 MAP kinase is identified as enhancer of RhoB up-regulation. Finally, RhoB is found to regulate TcdB-induced apoptosis. |