Protein Liquid Chromatography: Volume 61 Contributor(s): Kastner, M. (Author) |
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ISBN: 0444502114 ISBN-13: 9780444502117 Publisher: Elsevier Science OUR PRICE: $306.90 Product Type: Paperback - Other Formats Published: December 1999 |
Additional Information |
BISAC Categories: - Science | Chemistry - Organic - Science | Chemistry - Analytic - Science | Chemistry - Inorganic |
Dewey: 547.750 |
LCCN: 99052197 |
Series: Journal of Chromatography Library |
Physical Information: 2.2" H x 6" W x 9.2" (2.90 lbs) 933 pages |
Descriptions, Reviews, Etc. |
Publisher Description: Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on Getting started now and Where to go from here attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area. |